Colonic mucosal gene expression in irritable bowel syndrome rats by the liquid chip technology

Background: Irritable bowel syndrome (IBS) is one of the most frequent GI disorders. The etiology and pathogenesis underlying IBS are currently unclear. Gene influence a number of chronic disease processes and may also be involved in regulating disease activity in gastrointestinal disorders, but few studies of IBS have focused on gene expression. Objective: The objective of this study was to screen the differentially expressed colonic mucosal genes in IBS rats to build the expression profile of genes in the colon of IBS rats. Methods: Twenty SD rats were divided randomly into two groups: the rats of control group were normal rats; the rats of model group were induced by conditioned stimulus and unconditioned stimulus. The rats’ visceral sensitivity was evaluated by abdominal withdraw reaction. Then we screened differential expression of colonic mucosal gene by the liquid chip technology and verified by RT-PCR technology. Results: The IBS model was successfully established. Compared with control group, the dose of injection water was decreased in model group (P<0.01). We screened htr4, htr1a, 2rl3, nos1, Calca, npy, crhr2, il1b, p2rx3, nos2, tph1, crhr1, hmox1, trpv1, Vip, f2rl, tgfb1, htr3a, slc6a4, tff2, aqp8 from the colon, but we found that only the expression of nos1, il1b, htr3a in model group was up-regulated (P<0.05). Conclusion: Colonic mucosal genes may be involved in the pathogenesis of IBS with the regulation in colon function.